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For example, successful efforts to develop ligands targeting the orthosteric (glutamate) binding site of mGlu receptors have produced amino acid analogs, which often do not possess pharmacokinetic properties suitable for human dosing (Conn et al., 2014

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High-performance liquid chromatography (HPLC) was used to measure dopamine, metabolites of dopamine such as 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), ascorbic acid, and dehydroascorbic acid in the striatal tissue
