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This rapid metabolism, combined with poor water solubility and limited absorption in the gastrointestinal tract, leads to insufficient plasma concentrations to exert significant pharmacological effects 18,19

In this reaction, sedoheptulose 7-phosphate binds with the enzyme, and the TPP cofactor is activated to form a carbanion

Recordings were obtained using a Multiclamp 700B amplifier (Molecular Devices, Sunnyvale, CA), Analog signals were low-pass Bessel filtered at 2 kHz, digitized at 10 kHz through a Digidata 144 OA interface (Molecular Devices), and stored in a computer using Clampex 10.2 software (Molecular Devices)

see Table 4