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Zhang H, et al
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To prepare samples for OCR measurements, samples were prepared in mitochondrial assay buffer (70 mM sucrose, 220 mM mannitol, 10 mM KH2PO4, 5 mM MgCl2, 2 mM HEPES, 1 mM EGTA and 0.2% fatty acid-free BSA, pH 7.2) plus substrate (pyruvate and malate for the Complex I-mediated ETC activity assay, or succinate and rotenone for the Complex II-mediated ETC activity assay)
