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Barnett et al

The immature phase is when the plaque first forms, can be associated with pain, and may last 12 months whereby the plaque stabilizes

The wet dough was rubbed to small particles which size was proper for zebrafish, and then dried under 60 C

Ganguly P, Boserman P, van der Vegt NFA, Shea J-E (2018) Trimethylamine N-oxide counteracts urea denaturation by inhibiting proteinurea preferential interaction
