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Prediction of pI and net charge The EMBOSS iep software (EMBOSS) ( was used to calculate the theoretical pI and net charge at different pH values of unfused albumin variants and GLP-1 albumin fusions
The extraction of total RNA was conducted using the RNA extraction reagent Isogen (Nippon Gene), and the PCR reaction was performed as described before (Kobayashi et al., 2006)
The side effect I watch for most carefully isnt on either list
